Background Previous studies have suggested that exercise training improves cardiac autonomic drive in young and middleaged adults. In this study, we discuss the benefts for the elderly. Objectives We aimed to establish whether exercise still increases heart rate variability (HRV) beyond the age of 60 years, and to identify which training factors infuence HRV gains in this population. Methods Interventional controlled and non-controlled studies were selected from the PubMed, Ovid, Cochrane and Google Scholar databases. Only interventional endurance training protocols involving healthy subjects aged 60 years and over, and measuring at least one heart rate global or parasympathetic index, such as the standard deviation of the normal-to-normal intervals (SDNN), total frequency power (Ptot), root mean square of successive diferences between adjacent NN intervals (RMSSD), or high frequency power (HF) before and after the training intervention, were included. HRV parameters were pooled separately from short-term and 24 h recordings for analysis. Risks of bias were assessed using the Methodological Index for Non-Randomized Studies and the Cochrane risk of bias tool. A random-efects model was used to determine efect sizes (Hedges’ g) for changes, and heterogeneity was assessed using Q and I statistics. Results Twelve studies, seven of which included a control group, including 218 and 111 subjects, respectively (mean age 69.0±3.2 and 68.6±2.5), were selected for meta-analysis. Including the 12 studies demonstrated homogeneous signifcant efect sizes for short-term (ST)-SDNN and 24 h-SDNN, with efect sizes of 0.366 (95% CI 0.185–547) and 0.442 (95% CI 0.144–0.740), respectively. Controlled study analysis demonstrated homogeneous signifcant efect sizes for 24 h-SDNN with g=0.721 (95% CI 0.184–1.257), and 24 h-Ptot with g=0.731 (95% CI 0.195–1.267). Meta-regression analyses revealed positive relationships between ST-SDNN efect sizes and training frequency (Tau2 res=0.000; I2 res=0.000; p=0.0462). Conclusion This meta-analysis demonstrates a positive efect of endurance-type exercise on autonomic regulation in older adults. However, the selected studies expressed some risks of bias. We conclude that chronic endurance exercise leads to HRV improvements in a linear frequency–response relationship, encouraging the promotion of high-frequency training programmes in older adults.