Background: Physical Activity (PA) programs have been suggested to lower absenteeism due to illness in sedentary employees. The aim of this review was to examine available scientific literature in order to study PA effects in workplace absenteeism taking the program design into consideration. Methods: A search through 4 databases (Medline, Sportdiscus, Web of Science and Embase), from inception to December 2017, was conducted to identify control intervention and observational studies about PA and absenteeism published in either English or Spanish language using PRISMA procedures. Results: A total of 10 published studies between 1981 and 2017 met the inclusion criteria. Evidence from the review suggested that PA is effective in reducing illness-related absence. Likelihood of being off from workplace reported with sedentary workers reach more probabilities when compared with exercisers. Conclusions: General PA is considered paramount in order to lower employees' absenteeism, which could be more related to vigorous training. However, there is a lack of rigorous and more specific studies about the issue. More focused on PA effect on absenteeism, adjusted by intervention variables, research would be desirable.