Aim: This study aimed to evaluate the efficacy of music therapy for reducing the anxiety and pain of patients who underwent the biopsy. Background: Music can affect human anxiety and pain by triggering a neuroendocrine effect. Clinical study results indicated that music can influence the anxiety and pain caused by invasive procedures. There is no effective solution for anxiety and pain arising from a biopsy. Although researchers in this field have different views, music still holds promise in reducing the anxiety and pain in patients undergoing the biopsy. Design: Systematic review and meta-analysis of randomized controlled trials. Data Sources: Systematic searches were conducted in Pubmed, Embase, Medline and Cochrane databases for studies reported in the English language. The review period covered 2000 - December 2016. The outcome measure of interest was anxiety and pain