Mindfulness-based intervention is proposed and practiced as an alternative treatment, however, current evidence supporting the treatment effect of mindfulness-based interventions remains unclear. The study proposed to evaluate the effectiveness of mindfulness- based interventions for insomnia. Methods: Eight databases (including PubMed, Medline and PsycInfo) were searched and the reference list of relevant records were screened for identifying eligible studies. Randomized controlled trials examining any forms of mindfulness-based intervention which containing meditation will be included for optimizing the search. Clinical trials with very small sample were excluded. Statistical analyses were performed using Review Manager version 5.3 using random effect model. The quality of included studies were assessed according to the Cochrane Collaboration tool for assessing risk of bias. Results: An initial search result of 7462 records was then reduced to a final sample of fourteen selected trials for meta-analyses. A sampling of 1295 participant with sleep complaint was recruited, aged 57.5 in average and varied in health conditions. Subgroup analyses were conducted for the substantial heterogeneity among studies noted. Significant improvements were found in subjective insomnia severity when compared with no treatment/usual care in both healthy sample (SMD -0.78, 95% CI -0.99 to -0.57, p<0.001, 388 subjects) and cancer sample (SMD -0.25, 95% CI -0.40 to -0.10, p=0.001, 678 subjects). Inconsistent results were found in the specific sleep parameters measured by sleep diary and actigraph. However, insufficient data were available for comparing the efficacy of mindfulness-based intervention with other comparators, such as sleep hygiene education, psychotherapy and western medication. Conclusion: Despite the mindfulness-based interventions demonstrate clinical benefit for improving the subjective sleep quality, the validity of evidence was limited for the diversity of treatment contents, heterogeneity of sample, the small sample size of included trials and cultural influence.